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KMID : 0380019950100020159
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Korean Journal of Biotechnology and Bioengineering
1995 Volume.10 No. 2 p.159 ~ p.165
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Continuous Production Process of Methyl Fructoside Using Alginate-enclosed Microspheres
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Abstract
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Methyl fructoside was continuously produced in suspended bed enzyme reactor using alginate-enclosed microspheres biocatalyst which was developed for enzymatic synthesis of methyl fructoside. And the continuous operating conditions were optimized with reactor simulation in order to demonstrate a feasibility of commercialization of the continuous enzymatic production process development. The yield and productivity of methyl fructoside were as high as 47.1 % and 2g/P -h;respectively. The optimum operating conditions were pH 4.8, 30%(v/v) of methanol content and 2U/mB of enzyme activity when the initial concentration of sucrose is 0.291mol/. at the reaction temperature of 251C.
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